Animal and Genetic Engineering
The primary purpose of the Animal and Genetic Engineering Shared Resource is to provide researchers with facilities for housing and maintaining laboratory animals. Experienced staff perform all aspects of animal husbandry and assist researchers with specialized procedures and protocol development. The Animal Shared Resource is fully accredited by the Association for Assessment and Accreditation of Laboratory Animal Care (AAALAC) and animals are maintained in accordance with the applicable USDA regulations and the National Research Council’s Guide for the Care and Use of Laboratory Animals.
The Shared Resource also provides services for construction and production of genetically engineered mouse models.
Shared Resources Staff
Director and Attending Vet:
Rachel Strittmatter, DVM
Director of Husbandry & Operations:
Leyi Li, Ph.D.
The Animal Shared Resource staff performs a variety of services to assist investigators using laboratory animals. This care includes daily observation of animals for signs of disease, maintaining sanitary conditions of laboratory animal housing, and assistance in the treatment of animal health- related issues. Detailed services are below:
- Blood Collections
- Surgical Manipulations
- Cesarean Re-derivation
- Protocol Assistance
- Husbandry Management
- Investigator Orientation Programs
- Investigator Training Programs
- Importation and Exportation of Mutant Mice
- Diagnostic Services
- Tail Biopsy
- DNA Extraction
- Identification including tattoo or ear punching
- Mouse Breeding Colony Management
The Core generates transgenic or chimeric mice, and offers cryopreservation of both mouse embryos and sperm for storage and management of transgenic mouse lines. In addition to these standard services, the facility assists investigators in experiments that require sophisticated embryo manipulation or cell/embryo microinjection techniques. Reagents include:
Transgenic Mouse Services
- Production of transgenic mice by pronuclear injection
- Generation of chimeric mice by injection of genetically altered ES cells
Cell Culture Services
- Gene targeting in embryonic stem cells
- Derivation of ES cell lines from transgenic or mutant mice
- Maintenance of ES cell line stocks for use in gene targeting experiments
- Karyotyping of ES cells
- In vitro fertilization (IVF)
- Intracytoplasmic sperm injection (ICSI)
- Embryo and sperm cryopreservation
- Recovery of frozen embryos
- Rederivation of SPF mouse lines
- Consulting and technical assistance
New in vivo CRISPR Services
The Gene Targeting Core has established CRISPR mouse zygotic injection protocols that can be used to generate knock-in, knock-out and point mutations. This method cuts 2-3 months off the time to generate animals, as it eliminates the requirement of developing a targeted cell line in vitro. The Core is now working on protocols adopting the two component (crRNA and tracrRNA) strategy for guide RNA instead of a single guide RNA for CRISPR injection; this two component format makes synthesis of the guide RNA much easier.
Interested in using our new service? Contact Leyi Li at firstname.lastname@example.org or 516-422-4085.