Research Associate Professor/Director, Functional Genomics
Ph.D., Stony Brook University, 2002
email@example.com | 516-367-5418
RNA interference (RNAi) and CRISPR are widely used to functionally investigate mammalian genomes. It is our goal to develop and optimize these gene perturbation platforms to improve their effectiveness in understanding the biology of diseases.
Loss-of-function genetics has proven essential for interrogating the functions of genes and for probing their roles within the complex circuitry of biological pathways. Kenneth Chang developed genetic approaches for perturbing genes using shRNAs (RNAi), optimized the effectiveness of these technologies, and created shRNA infrastructure and resources for genetic screens in mammalian cells. These resources include shRNA libraries modeled after microRNAs (miRNAs), algorithms for predicting highly potent shRNA designs. Chang has performed many genetic screens in cancer cells and has expanded his research to include a significant focus on CRISPR technologies and their applications (e.g. organoid systems).
As the Director of the Functional Genomics Shared Resource, Chang provides access to advanced RNAi/CRISPR technologies, construction of custom RNAi/CRISPR libraries, and assists in the design and implementation of genetic screens to support research programs at CSHL.