Tissue Culture
For many types of research, tissue culture offers the ideal model system: rapid, cost effective, and easy to genetically manipulate. The CSHL Tissue Culture Facility offers affordable access to hundred of cell lines, with many common stocks being actively cultured and readily available. Several specialized tissue culture rooms are available and staffed across campus to support the needs of researchers and provide training. In addition, mycoplasma testing and cell-line authentication is coordinated through this facility.
Staff
Resource Head
James TC Manager
Margaret Falkowski
516-367-6821
falkowsk@cshl.edu
James Building Manager
Eleni Boikoglou
516-367-8494
eboikogl@cshl.edu
Hillside TC Manager
Julie Cheong
516-367-6860
jcheong@cshl.edu
Hillside Laboratory Building Manager/Cell-Line Validation Coordinator
Amy Brady
516-367-5090
abrady@cshl.edu
CSHL offers multiple specialized tissue culture rooms (access restricted to trained users):
Several buildings have their own TC rooms which are managed locally. In addition, the James and Hillside facilities have additional specialized facilities available for CSHL use (pending availability). These are:
BL2+ virus room (both James and Hillside); restricted to lab personnel who have completed required biosafety training conducted by EH&S; for access, contact Eleni Boikoglou (James) or Julie Cheong (Hillside).
Embryonic Stell Cell room: used for manipulation of ES cells; for access, contact Eleni Boikoglou (James) or Julie Cheong (Hillside).
The Tissue Culture Facility offers affordable and rapid access to a large number of cell lines and stocks. Services include:
Stock Cell Lines: Standard cell lines (HeLa, 293, NIH 3T3,U2OS and MCF-7) are actively growing at all times. Plates are available to anyone on campus. Contact Margaret Falkowski.
Tissue Culture Repository: An extensive collection of cell lines is stored frozen in liquid nitrogen and are available upon request. Contact Margaret Falkowski.
Suspension cells stocks: Culture of HeLa and 293T cells can be scaled to large volumes (500 mls to 20 liters) as requested. Contact Margaret Falkowski.
Mycoplasma Testing: All new cell lines arriving at the lab should be tested for mycoplasma. Facility offers testing of these and any other lines as needed. Contact Margaret Falkowski for James; Julie Cheong for Hillside.
Serum Testing: Fetal Bovine Serum is tested in individual lots to ensure that they support support the growth of a panel of cell lines. The verified lots of FBS can be purchased through the Freezer Program.
Cell Line Authentication: Cell line misidentification is a ubiquitous problem in biological research. As a result, new NIH guidelines require that investigators ensure the “identity and validity of key biological and/or chemical resources.” To help researchers comply with these regulations, CSHL now offers cell line authentication through STR profiling. Contact Amy Brady to learn more.
Training & Consulting: The facility offers information, instruction, and assistance on all topics related to tissue culture.