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MAPseq (Multiplexed Analysis of Projections by Sequencing) is a novel high-throughput method for brain mapping. It leverages next-generation sequencing to allow massive multiplexing of long-range projections, from thousands of cells, at single neuron resolution, from a single brain. This method was first described by Anthony Zador’s team (Kebschull et al, 2016 “High-throughput mapping of single neuron projections by sequencing of barcoded RNA”).

Core Facility Staff

Resource Head

Anthony Zador, M.D., Ph.D.

Research Investigator

Ching/Huiqing Zhan, Ph.D.

Core Technicians

Yan Li
Nicole Gemmill

MAPseq can be used to:

  • Quantify the projections of thousands—or even millions—of individual neurons in parallel within a single brain.
  • Produce multiple projection patterns from one to several injection sites, at single-neuron resolution.
  • Determine the brain-wide projections from a given area within one month.
  • Compare projection patterns originating from different areas of the brain.
  • Determine whether individual neurons from a given area project to a specific area of interest.
  • Perform single-neuron tracing in less common animal model systems, including rodents and potentially non-human primates.

This core provides reagents and protocols for single neuron labeling, and helps users in processing tissue samples for high throughput sequencing. Services include:

  • Sindbis virus barcode library preparation: Providing Sindbis virus library containing millions of barcodes.
  • Sample processing: RNA extraction, Reverse Transcription and PCR to make the library for Next Generation Sequencing.
  • Next Generation Sequencing: Submitting the library to CSHL Next Generation Sequencing Core Facility for PE36 NextSeq run.
  • Preliminary sequencing data analysis: Analyzing the Next Generation Sequencing raw data and generating Barcode Marix (the abundance of each barcoded neuron in each tissue sample).