MAPseq (Multiplexed Analysis of Projections by Sequencing) is a novel high-throughput method for brain mapping. It leverages next-generation sequencing to allow massive multiplexing of long-range projections, from thousands of cells, at single neuron resolution, from a single brain. This method was first described by Anthony Zador’s team (Kebschull et al, 2016 “High-throughput mapping of single neuron projections by sequencing of barcoded RNA”).
This core provides reagents and protocols for single neuron labeling, and helps users in processing tissue samples for high throughput sequencing. Services include:
- Sindbis virus barcode library preparation: Providing Sindbis virus library containing millions of barcodes.
- Sample processing: RNA extraction, Reverse Transcription and PCR to make the library for Next Generation Sequencing.
- Next Generation Sequencing: Submitting the library to CSHL Next Generation Sequencing Core Facility for PE36 NextSeq run.
- Preliminary sequencing data analysis: Analyzing the Next Generation Sequencing raw data and generating Barcode Marix (the abundance of each barcoded neuron in each tissue sample).