MAPseq (Multiplexed Analysis of Projections by Sequencing) is a novel high-throughput method for brain mapping. It leverages next-generation sequencing to allow massive multiplexing of long-range projections, from thousands of cells, at single neuron resolution, from a single brain. This method was first described by Anthony Zador’s team (Kebschull et al, 2016 “High-throughput mapping of single neuron projections by sequencing of barcoded RNA”).
MAPseq can be used to:
- Quantify the projections of thousands—or even millions—of individual neurons in parallel within a single brain.
- Produce multiple projection patterns from one to several injection sites, at single-neuron resolution.
- Determine the brain-wide projections from a given area within one month.
- Compare projection patterns originating from different areas of the brain.
- Determine whether individual neurons from a given area project to a specific area of interest.
- Perform single-neuron tracing in less common animal model systems, including rodents and potentially non-human primates.
This core provides reagents and protocols for single neuron labeling, and helps users in processing tissue samples for high throughput sequencing. Services include:
- Sindbis virus barcode library preparation: Providing Sindbis virus library containing millions of barcodes.
- Sample processing: RNA extraction, Reverse Transcription and PCR to make the library for Next Generation Sequencing.
- Next Generation Sequencing: Submitting the library to CSHL Next Generation Sequencing Core Facility for PE36 NextSeq run.
- Preliminary sequencing data analysis: Analyzing the Next Generation Sequencing raw data and generating Barcode Marix (the abundance of each barcoded neuron in each tissue sample).