MAPseq
MAPseq (Multiplexed Analysis of Projections by Sequencing) is a novel high-throughput method for brain mapping. It leverages next-generation sequencing to allow massive multiplexing of long-range projections, from thousands of cells, at single neuron resolution, from a single brain. This method was first described by Anthony Zador’s team (Kebschull et al, 2016 “High-throughput mapping of single neuron projections by sequencing of barcoded RNA”).
Core Facility Staff
Resource Head
Anthony Zador, M.D., Ph.D.
zador@cshl.edu
Research Investigator
Ching/Huiqing Zhan, Ph.D.
hzhan@cshl.edu
Core Technicians
Yan Li yali@cshl.edu
Nicole Gemmill gemmill@cshl.edu
MAPseq can be used to:
- Quantify the projections of thousands—or even millions—of individual neurons in parallel within a single brain.
- Produce multiple projection patterns from one to several injection sites, at single-neuron resolution.
- Determine the brain-wide projections from a given area within one month.
- Compare projection patterns originating from different areas of the brain.
- Determine whether individual neurons from a given area project to a specific area of interest.
- Perform single-neuron tracing in less common animal model systems, including rodents and potentially non-human primates.
This core provides reagents and protocols for single neuron labeling, and helps users in processing tissue samples for high throughput sequencing. Services include:
- Sindbis virus barcode library preparation: Providing Sindbis virus library containing millions of barcodes.
- Sample processing: RNA extraction, Reverse Transcription and PCR to make the library for Next Generation Sequencing.
- Next Generation Sequencing: Submitting the library to CSHL Next Generation Sequencing Core Facility for PE36 NextSeq run.
- Preliminary sequencing data analysis: Analyzing the Next Generation Sequencing raw data and generating Barcode Marix (the abundance of each barcoded neuron in each tissue sample).
Orders can be placed through the iLab Solutions MapSeq core
Rates
Rates last updated: Feb 2020
| Service | Unit | External |
|---|---|---|
|
Virus library – sufficient for 96 injections:
~20M barcodes (24 vials, 4 ul ea)
Test virus with single barcodes (2 vials, 3ul ea)
|
per library | $1,570 |
| Sample Processing | 12 samples | $1,890 |
| 24 samples | $3,360 | |
| 48 samples | $5,350 | |
| 96-well plate | $8,230 | |
| Sequencing (NextSeq high output PE36) | per library | $2,360 |
| Data analysis | per library | $1,570 |
| Total project (sample processing, sequencing, & analysis) | 12 samples | $5,820 |
| 24 samples | $7,290 | |
| 48 samples | $9,280 | |
| 96-well plate | $12,160 |