Paolo Cifani
Research Assistant Professor/Director, Mass Spectrometry Shared Resource
Cancer Center Member
Ph.D., Lund University, 2013
cifani@cshl.edu | 516-367-6806
We develop innovative mass spectrometry-based approaches to measure how protein activities are regulated under physiologic conditions and in pathological states.
The vast majority of cellular biochemical processes are mediated by the coordinated activities of proteins. Protein activities are in turn controlled by their concentration and localization, by chemical modification of specific amino acids, by interaction with other biomolecules, and by several other mechanisms. Different cellular functional states, including those observed in cancer and other diseases, are almost invariably defined by specific patterns of protein regulation. The Cifani lab develops innovative approaches to study protein regulation using high-resolution mass spectrometry and liquid chromatography. Using these methods, we aim to reveal differences in concentration, structure, and chemical modification of cellular proteins from minimal sample amounts, such as those obtained from clinical specimens.
All Publications
Aldehydes alter TGF-β signaling and induce obesity and cancer
31 Aug 2024 | Cell Reports | 43(9):114676
Yang, Xiaochun; Bhowmick, Krishanu; Rao, Shuyun; Xiang, Xiyan; Ohshiro, Kazufumi; Amdur, Richard; Hassan, Md; Mohammad, Taj; Crandall, Keith; Cifani, Paolo; Shetty, Kirti; Lyons, Scott; Merrill, Joseph; Vegesna, Anil; John, Sahara; Latham, Patricia; Crawford, James; Mishra, Bibhuti; Dasarathy, Srinivasan; Wang, Xin; Yu, Herbert; Wang, Zhanwei; Huang, Hai; Krainer, Adrian; Mishra, Lopa;
The molecular basis of Human FN3K mediated phosphorylation of glycated substrate
5 Aug 2024 | bioRxiv
Garg, Ankur; On, Kin; Xiao, Yang; Elkayam, Elad; Cifani, Paolo; David, Yael; Joshua-Tor, Leemor;
MARK2/MARK3 kinases are catalytic co-dependencies of YAP/TAZ in human cancer
26 Jul 2024 | Cancer Discovery
Klingbeil, Olaf; Skopelitis, Damianos; Tonelli, Claudia; Yoshimoto, Toyoki; Alpsoy, Aktan; Panepinto, Maria; Minicozzi, Francesca; Merrill, Joseph; Cafiero, Amanda; Aggarwal, Disha; Russo, Suzanne; Ha, Taehoon; Demerdash, Osama; Wee, Tse-Luen; Spector, David; Lyons, Scott; Tuveson, David; Cifani, Paolo; Vakoc, Christopher;
SRSF1 interactome determined by proximity labeling reveals direct interaction with spliceosomal RNA helicase DDX23
21 May 2024 | Proceedings of the National Academy of Sciences of the United States of America | 121(21):e2322974121
Segovia, Danilo; Adams, Dexter; Hoffman, Nickolas; Safaric Tepes, Polona; Wee, Tse-Luen; Cifani, Paolo; Joshua-Tor, Leemor; Krainer, Adrian;
MARK2/MARK3 kinases are catalytic co-dependencies of YAP/TAZ in human cancer
28 Feb 2024 | bioRxiv
Klingbeil, Olaf; Skopelitis, Damianos; Tonelli, Claudia; Alpsoy, Aktan; Minicozzi, Francesca; Aggarwal, Disha; Russo, Suzanne; Ha, Taehoon; Demerdash, Osama; Spector, David; Tuveson, David; Cifani, Paolo; Vakoc, Christopher;
SETD1A function in leukemia is mediated through interaction with mitotic regulators BuGZ/BUB3
9 Oct 2023 | EMBO Reports | 24(10):e57108
Perlee, Sarah; Kikuchi, Sota; Nakadai, Tomoyoshi; Masuda, Takeshi; Ohtsuki, Sumio; Matsumoto, Makoto; Rahmutulla, Bahityar; Fukuyo, Masaki; Cifani, Paolo; Kentsis, Alex; Roeder, Robert; Kaneda, Atsushi; Hoshii, Takayuki;
Plasma cells in human pancreatic ductal adenocarcinoma secrete antibodies to self-antigens
26 Sep 2023 | JCI insight | :e172449
Yao, Min; Preall, Jonathan; Yeh, Johannes; Pappin, Darryl; Cifani, Paolo; Zhao, Yixin; Shen, Sophia; Moresco, Philip; He, Brian; Patel, Hardik; Habowski, Amber; King, Daniel; Raphael, Kara; Rishi, Arvind; Sejpal, Divyesh; Weiss, Matthew; Tuveson, David; Fearon, Douglas;
Integrative Proteogenomics Using ProteomeGenerator2.
4 Aug 2023 | Journal of Proteome Research | 22(8):2750-2764
Kwok, Nathaniel; Aretz, Zita; Takao, Sumiko; Ser, Zheng; Cifani, Paolo; Kentsis, Alex;
RNA binding protein SYNCRIP maintains proteostasis and self-renewal of hematopoietic stem and progenitor cells
21 Apr 2023 | Nature Communications | 14(1):2290
Herrejon Chavez, Florisela; Luo, Hanzhi; Cifani, Paolo; Pine, Alli; Chu, Karen; Joshi, Suhasini; Barin, Ersilia; Schurer, Alexandra; Chan, Mandy; Chang, Kathryn; Han, Grace; Pierson, Aspen; Xiao, Michael; Yang, Xuejing; Kuehm, Lindsey; Hong, Yuning; Nguyen, Diu; Chiosis, Gabriela; Kentsis, Alex; Leslie, Christina; Vu, Ly; Kharas, Michael;
Integrative proteogenomics using ProteomeGenerator2.
4 Jan 2023 | bioRxiv
Kwok, Nathaniel; Aretz, Zita; Takao, Sumiko; Ser, Zheng; Cifani, Paolo; Kentsis, Alex;