Jon Preall

Jon Preall

Research Associate Professor/Head of Genomics Technology Development
Cancer Center Member

Ph.D., Northwestern University, 2009

Faculty Profile

Developing single-cell genomics technologies for applications related to cancer progression, immune surveillance, and discovery of rare novel cell types and transcriptional programs.

Biology is inescapably a study of diversity. Understanding complex biological systems, both in health and in disease, requires the deconvolution of a heterogeneous milieu into its constituent parts. New experimental tools have made it possible to investigate gene expression, chromatin architecture, and genetic alterations in thousands of single cells simultaneously. These technologies have revolutionized how we approach biological problems by granting the ability to measure interactions within the diverse cellular mixture comprising a tumor microenvironment, a developing stem cell niche, a complex organ such as the brain, or an immune response. The Preall Lab deploys and develops single-cell technologies and other high-throughput genomics workflows to explore biological diversity at high resolution. Using single-cell transcriptome profiling, the lab has characterized fibroblast populations that coordinate tissue remodeling within pancreatic tumors and govern interactions between cancerous cells, signals driving their growth, and surveillance by the immune system. The Preall lab is also developing technology to push single cell capabilities towards larger and larger cell numbers to enable massive scale studies of the immune repertoire across patient cohorts and over time.

All Publications

A mucus production programme promotes classical pancreatic ductal adenocarcinoma

23 Jan 2024 | Gut
Tonelli, Claudia, Yordanov, Georgi, Hao, Yuan, Deschênes, Astrid, Hinds, Juliene, Belleau, Pascal, Klingbeil, Olaf, Brosnan, Erin, Doshi, Abhishek, Park, Youngkyu, Hruban, Ralph, Vakoc, Christopher, Dobin, Alexander, Preall, Jonathan, Tuveson, David

ROR2 regulates cellular plasticity in pancreatic neoplasia and adenocarcinoma.

14 Dec 2023 | bioRxiv
Benitz, Simone, Steep, Alexander, Nasser, Malak, Preall, Jonathan, Mahajan, Ujjwal, McQuithey, Holly, Loveless, Ian, Davis, Erick, Wen, Hui-Ju, Long, Daniel, Metzler, Thomas, Zwernik, Samuel, Louw, Michaela, Rempinski, Donald, Salas-Escabillas, Daniel, Brender, Sydney, Song, Linghao, Huang, Ling, Zhang, Zhenyu, Steele, Nina, Regel, Ivonne, Bednar, Filip, Crawford, Howard

Plasma cells in human pancreatic ductal adenocarcinoma secrete antibodies to self-antigens

26 Sep 2023 | JCI insight | :e172449
Yao, Min, Preall, Jonathan, Yeh, Johannes, Pappin, Darryl, Cifani, Paolo, Zhao, Yixin, Shen, Sophia, Moresco, Philip, He, Brian, Patel, Hardik, Habowski, Amber, King, Daniel, Raphael, Kara, Rishi, Arvind, Sejpal, Divyesh, Weiss, Matthew, Tuveson, David, Fearon, Douglas

Myo-differentiation reporter screen reveals NF-Y as an activator of PAX3-FOXO1 in rhabdomyosarcoma

5 Sep 2023 | Proceedings of the National Academy of Sciences of the United States of America | 120(36):e2303859120
Sroka, Martyna, Skopelitis, Damianos, Vermunt, Marit, Preall, Jonathan, El Demerdash, Osama, de Almeida, Larissa, Chang, Kenneth, Utama, Raditya, Gryder, Berkley, Caligiuri, Giuseppina, Ren, Diqiu, Nalbant, Benan, Milazzo, Joseph, Tuveson, David, Dobin, Alexander, Hiebert, Scott, Stengel, Kristy, Mantovani, Roberto, Khan, Javed, Kohli, Rahul, Shi, Junwei, Blobel, Gerd, Vakoc, Christopher

Cystatin C is glucocorticoid responsive, directs recruitment of Trem2+ macrophages, and predicts failure of cancer immunotherapy

9 Aug 2023 | Cell Genomics | 3(8):100347
Kleeman, Sam, Thakir, Tuba, Demestichas, Breanna, Mourikis, Nicholas, Loiero, Dominik, Ferrer, Miriam, Bankier, Sean, Riazat-Kesh, Yosef, Lee, Hassal, Chantzichristos, Dimitrios, Regan, Claire, Preall, Jonathan, Sinha, Sarthak, Rosin, Nicole, Yipp, Bryan, de Almeida, Luiz, Biernaskie, Jeff, Dufour, Antoine, Tober-Lau, Pinkus, Ruusalepp, Arno, Bjorkegren, Johan, Ralser, Markus, Kurth, Florian, Demichev, Vadim, Heywood, Todd, Gao, Qing, Johannsson, Gudmundur, Koelzer, Viktor, Walker, Brian, Meyer, Hannah, Janowitz, Tobias

Practical Considerations for Single-Cell Genomics

Aug 2022 | Current Protocols | 2(8):e498
Regan, Claire, Preall, Jonathan

OCA-T1 and OCA-T2 are coactivators of POU2F3 in the tuft cell lineage

16 May 2022 | Nature
Wu, Xiaoli, He, Xue-Yan, Ipsaro, Jonathan, Huang, Yu-Han, Preall, Jonathan, Ng, David, Shue, Yan, Sage, Julien, Egeblad, Mikala, Joshua-Tor, Leemor, Vakoc, Christopher

Single-cell genomic analysis of triple-negative breast cancer fibroblasts uncovers evolutionarily conserved features and potential therapeutic targets

2022 | bioRxiv
Delgado, Ana, Nemajerova, Alice, Rao, Manisha, Li, Jinyu, Marchenko, Natalia, Preall, Jonathan, Moll, Ute, Egeblad, Mikala, Powers, Scott

Patient-derived triple-negative breast cancer organoids provide robust model systems that recapitulate tumor-intrinsic characteristics

18 Feb 2022 | Cancer Research
Bhatia, Sonam, Kramer, Melissa, Russo, Suzanne, Naik, Payal, Arun, Gayatri, Brophy, Kyle, Andrews, Peter, Fan, Cheng, Perou, Charles, Preall, Jonathan, Ha, Taehoon, Plenker, Dennis, Tuveson, David, Rishi, Arvind, Wilkinson, John, McCombie, W, Kostroff, Karen, Spector, David

Oral famotidine versus placebo in non-hospitalised patients with COVID-19: a randomised, double-blind, data-intense, phase 2 clinical trial

10 Feb 2022 | Gut
Brennan, Christina, Nadella, Sandeep, Zhao, Xiang, Dima, Richard, Jordan-Martin, Nicole, Demestichas, Breanna, Kleeman, Sam, Ferrer, Miriam, von Gablenz, Eva, Mourikis, Nicholas, Rubin, Michael, Adnani, Harsha, Lee, Hassal, Ha, Taehoon, Prum, Soma, Schleicher, Cheryl, Fox, Sharon, Ryan, Michael, Pili, Christina, Goldberg, Gary, Crawford, James, Goodwin, Sara, Zhang, Xiaoyue, Preall, Jonathan, Costa, Ana, Conigliaro, Joseph, Masci, Joseph, Yang, Jie, Tuveson, David, Tracey, Kevin, Janowitz, Tobias

See more publications