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Single-Cell Biology

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The Single-Cell Biology Shared Resource provides access to facilities, equipment, and expertise for the study of gene expression, protein abundance, and genetic alterations at single-cell resolution. We offer expert-level, quality-controlled service for challenging applications single cell applications, and can provide assistance with every stage of a single-cell experiment, including study design, tissue dissociation, cell culture, population enrichment, cell sorting, quality control, and library preparation, and data analysis.

To inquire about a single-cell project idea, contact Jon Preall.

EXTERNAL USERS: Many CSHL Core Facility Services are also available to external users, both academic and non-academic, and can be accessed through iLab. Please reach out to individual core directors for more information on services, rates and how to access.

Shared Resource Staff

Resource Head

Jessica Tollkuhn, Ph.D.
tollkuhn@cshl.edu

Head of Genomics Technology Development

Jonathan Preall, Ph.D.
516-422-4086
jpreall@cshl.edu

Staff

Claire Regan, Analyst
cregan@cshl.edu

Evan Zhang
ezhang@cshl.edu

Explore all the CSHL Core Facilities

Equipment and Services available:

    • 10X Genomics Chromium Platform

      • Single Cell 3’ Gene Expression
        With available add-ons:

        • Surface Protein Expression (aka. CITE-seq)
        • CRISPR Perturbation Screening
        • Targeted Gene Expression
      • Single Cell Immune Profiling
      • Single Cell ATAC-seq
      • Single Cell Multi-omics (ATAC + Gene Expression)
      • Single Cell CNV
    • Sony SH800 Cell Sorter
      • Bulk or single-cell sorting
      • 4 lasers (405nm, 488nm, 561nm, 640nm)
      • 6 detectors
      • 2-way, 96-well, and 385 well sorting with indexing capabilities
    • Fluidigm C1 Single-Cell autoprep system
      • 96- and 800- chamber Integrated Fluidic Circuits (IFCs)
      • Flexible downstream applications:
        • Full gene-body coverage RNAseq
        • ATAC-seq
        • Other custom workflows
    • 10X Genomics Visium Spatial Transcriptomics

Talk with us about pilot projects using spatial transciptomics

Have a project idea? Contact us!

We are currently seeking a limited number of beta-test partners to provide tissue samples along with an idea of the target cell subpopulations to be enriched prior to single cell capture and sequencing. If desired, the single-cell core facility will work closely with clients to develop optimal tissue isolation, handling, and dissociation protocols, as well as cell sorting. Contact Jon Preall to get started.

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Cold Spring Harbor Laboratory is an NCI-designated Cancer Center. As a basic research institution, CSHL does not treat patients. Information about individual cancers is available at the NCI CancerNet. Questions about CSHL’s cancer research program should be directed to our Communications Department.