DNALC Live: DNA Barcoding Part II: PCR and Gel Electrophoresis

Watch on 

[YouTube login required to chat]

Activity description:

DNA Barcoding, Part 2 (of 3)

DNA barcoding allows for the identification of an organism through analysis of a short region of DNA within its genome, similar to how the pattern of bars in a universal product code (UPC) identifies each consumer product. Follow Dr. Sharon Pepenella through this three-part series as she introduces you to the wet lab procedures behind isolating, amplifying, and sequencing a barcode region of an organism’s DNA to identify the species!

In this DNALC Live session, you will:

• Gain an understanding of how Polymerase Chain Reaction (PCR) is used to make many copies of a specific region of DNA

• Explore how gel electrophoresis is used to separate and visualize fragments of DNA

• Amplify and observe the DNA barcode region from the processed samples in Part I