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CSHL Cancer Center - Flow Cytometry (FACS)

Flow Cytometry is an essential tool that is widely used in cancer research. The Flow Cytometry Shared Resource provides equipment, training, and operating assistance for cell sorting and analysis. Facility staff oversees equipment maintenance, trains new users, and assists with assay development and operation of equipment. Outside users are welcome to contact the facility manager for appointments, rates, or more information about services.

Shared Resources Staff
Resource Head Manager Supervisor Technician
Douglas Fearon, M.D.  
Pamela Moody  
Linda Rodgers  
Sean D'Italia  

FACS can be used in a variety of applications including analysis of cell cycle progression and apoptosis, quantitation of cell surface-expressed receptors and other markers, sorting and analysis of GFP-or mCherry tagged cells in complex populations, and sorting and enrichment of stem cells from different tissues based on cell-surface markers or subpopulations of cancer cells based on aneuploidy. This Shared Resource offers:

  • Training in use of equipment
  • Consulting on experimental design
  • Operation of instruments
In addition, tissue culture facilities are available for maintenance of cells during experiments.

The Resource offers three different types of cell sorters, which can be used for anything from routine analytical procedures to separating cells from tissue samples. The instrumentation includes:

  • Becton Dickinson LSRII Cell Analyzer with 4 lasers:
    • 405 nm Violet
    • 488 nm Blue
    • 561 nm Yellow-Green
    • 633 nm Red
  • Becton Dickinson FACSAria I with 3 lasers
    • 405nm Violet
    • 488nm Blue
    • 633nm Red
  • Becton Dickinson FACSAria SORP with 5 lasers and automated cell deposition unit for sorting cells into 96-well plates.
    • 355nm UV
    • 405nm Violet
    • 488nm Blue
    • 561nM Yellow-Green
    • 640nm Red
NCIlogo Cold Spring Harbor Laboratory is an NCI-designated Cancer Center. As a basic research institution, CSHL does not treat patients. Information about individual cancers is available at the NCI CancerNet. Questions about CSHL's cancer research program should be directed to our Public Affairs Department.