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CSHL Cancer Center - DNA Sequencing

The DNA Sequencing Shared Resource provides access to high throughput Next Generation Sequencing (NGS) technologies and to full service Sanger DNA sequencing using fluorescence-based technology. In combination, the Resource offers cutting-edge technology along side convenient in-house services for a broad range of genetic analysis. For more information see below or contact the Resource Director, Dick McCombie, Ph.D.

Shared Resources Staff
Resource Head NGS Project Manager Capillary Sequencing Manager
W. Richard McCombie, Ph.D.  
Eric Antoniou  
Spencer Teplin  

The Woodbury genome center is home to the latest in both short and long read sequencing technology. For short reads, the HiSeqs can produce over 300 billion bases of sequence in eleven days while the Miseq can yield over 1.5 billion bases in 27 hours. The Pacific Biosciences RS instruments can generate medium size sequences at high accuracy (300-600 bases) or long sequences (1 to 4 Kbases) at an 85% accuracy. To ensure that this technology is widely accessible to the CSHL community, Eric Antoniou is available for consultation on applications, experimental set-up, and strategies for experimental design.

Sequencing Equipment

  • HiSeq 2000 (two)
  • HiSeq 2500
  • MiSeq (two)
  • NextSeq500
  • Pacific Biosciences Sequencer (two)


The DNA Sequencing Shared Resource provides cost effective and rapid DNA sequence analysis using an ABI3730 instrument. The DNA Sequencing Facility offers full service fluorescent-based capillary DNA sequencing. Users can submit samples of PCR products, single and double stranded plasmids, BACs and cosmids. Facility staff set up reactions, run the samples and post final data on a shared drive for retrieval. The resource also provides consulting expertise for DNA preparation to improve the DNA sequencing results of the users. The on-site service provides convenience and a rapid turn-around time, facilitating the many projects that require DNA sequencing support.

NCIlogo Cold Spring Harbor Laboratory is an NCI-designated Cancer Center. As a basic research institution, CSHL does not treat patients. Information about individual cancers is available at the NCI CancerNet. Questions about CSHL's cancer research program should be directed to our Public Affairs Department.