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CSHL Cancer Center - Animal and Genetic Engineering

The primary purpose of the Animal and Genetic Engineering Shared Resource is to provide researchers with facilities for housing and maintaining laboratory animals. Experienced staff perform all aspects of animal husbandry and assist researchers with specialized procedures and protocol development. The Animal Shared Resource is fully accredited by the Association for Assessment and Accreditation of Laboratory Animal Care (AAALAC) and animals are maintained in accordance with the applicable USDA regulations and the National Research Council’s Guide for the Care and Use of Laboratory Animals. The Shared Resource also provides services for construction and production of genetically engineered mouse models.

Shared Resource Staff
Resource Head Animal Facility Genetic Engineering
Alea Mills, PhD  
 
Director and Attending Vet:
Rachel Strittmatter, DVM 
Director, Husbandry and Operations:
Lisa Bianco  
Manager:
Jodi Coblentz  
Director:
Leyi Li, PhD  

The Animal Shared Resource staff performs a variety of services to assist investigators using laboratory animals. This care includes daily observation of animals for signs of disease, maintaining sanitary conditions of laboratory animal housing, and assistance in the treatment of animal health- related issues. Detailed services are below:

  • Immunizations
  • Blood Collections
  • Surgical Manipulations
  • Necropsies
  • Cesarean Re-derivation
  • Protocol Assistance
  • Husbandry Management
  • Investigator Orientation Programs
  • Investigator Training Programs
  • Importation and Exportation of Mutant Mice
  • Diagnostic Services
  • Tail Biopsy
  • DNA Extraction
  • Identification including tattoo or ear punching
  • Mouse Breeding Colony Management
The Core generates transgenic or chimeric mice, and offers cryopreservation of both mouse embryos and sperm for storage and management of transgenic mouse lines. In addition to these standard services, the facility assists investigators in experiments that require sophisticated embryo manipulation or cell/embryo microinjection techniques. Reagents include:

Transgenic Mouse Services
  • Production of transgenic mice by pronuclear injection
  • Generation of chimeric mice by injection of genetically altered ES cells

Cell Culture Services
  • Gene targeting in embryonic stem cells
  • Derivation of ES cell lines from transgenic or mutant mice
  • Maintenance of ES cell line stocks for use in gene targeting experiments
  • Karyotyping of ES cells

Other Services
  • In vitro fertilization (IVF)
  • Intracytoplasmic sperm injection (ICSI)
  • Embryo and sperm cryopreservation
  • Recovery of frozen embryos
  • Rederivation of SPF mouse lines
  • Consulting and technical assistance
NCIlogo Cold Spring Harbor Laboratory is an NCI-designated Cancer Center. As a basic research institution, CSHL does not treat patients. Information about individual cancers is available at the NCI CancerNet. Questions about CSHL's cancer research program should be directed to our Public Affairs Department.