Description The Gene Targeting Shared Resource assists with the construction and management of genetically altered mouse strains. The primary services provided by this shared resource are the generation of transgenic or chimeric mice. In addition, the experienced staff assist investigators with gene targeting experiments in mouse embryonic stem cells. Cryopreservation of both mouse embryos and sperm is offered for storage and management of transgenic mouse lines.

Services
•	Gene targeting in embryonic stem (ES) cells
•	Generation of transgenic mice by pronuclear injection
•	Generation of chimeric mice by injection of genetically altered ES cells
•	Maintenance of ES cell line stocks for use in gene targeting experiments
•	Mouse embryo re-derivation services
•	Cryopreservation of embryos and sperm
•	Recovery of cryopreserved embryos
•	SPF mouse rederivation
•	In vitro fertilization (IVF)
•	Technical assistance with sophisticated embryo manipulation or cell/embryo microinjection techniques

New services (coming soon):
•	Intracytoplasmic sperm injection
•	ES cell chimeras using tetraploid embryo host
•	Gene knockdowns in mice using shRNA constructs

Links Generation of constitutive or conditional gene knockouts may be facilitated by use of available libraries of gene targeting vectors or interfering constructs. Such resources greatly speed up generation of mouse models with defined germline mutations.

Gene Targeting Libraries
•	Mouse Gene Targeting Library
•	CSHL RNA Interference library
•	The German Gene Trap Consortium (GGTC)
•	The International Gene Trap Consortium (IGTC)
•	The Gene Trap Consortium