Antibody Shared Resource
| Resource Head | Manager | Technician |
| Adrian Krainer, Ph.D. | Carmelita Bautista 516.367.8819 This e-mail address is being protected from spambots. You need JavaScript enabled to view it |
Margaret Falkowski |
DESCRIPTION
The Antibody Shared Resource provides a central site for the production of monoclonal and polyclonal antibodies for research use. Its mission is to provide the resources and expertise to generate these reagents in a highly efficient and cost-effective manner. In the case of monoclonal antibodies, CSHL Cancer Center scientists provide specific antigens, and the Shared Resource personnel handle all stages of immunization, fusion, primary screening of hybridomas, cell culture, single-cell cloning, freezing and storage of hybridoma lines, and large-scale production of monoclonal antibodies in cell culture or in vivo. Specialized monoclonal antibody screens are coordinated with individual scientists. Polyclonal antibody production is outsourced, but the Shared Resource personnel are in charge of processing and distribution of samples. The Shared Resource also tests and implements new techniques, reagents, and assay improvements related to the different stages of antibody production.
SERVICESComplete Hybridoma Development
- Immunization
- Cell Fusion
- Screening
- Cloning and amplification
Monoclonal Antibody Production
- In vivo ascites
- In vitro ascites
- Cell culture supernatants
Isotyping
Storage and archiving of hybridoma lines
Polyclonal services are outsourced to Covance or to Primm
