DESCRIPTION
Flow Cytometry is an essential tool used widely for a variety of applications including analysis of cell cycle progression and apoptosis, quantitation of cell surface-expressed receptors and other markers, sorting and analysis of GFP-or mCherry tagged cells in complex populations, and sorting and enrichment of stem cells from different tissues based on cell-surface markers or subpopulations of cancer cells based on aneuploidy.

The Flow Cytometry Core Facility provides equipment, training and operating assistance for cell sorting and analysis. Facility staff oversee equipment maintenance, train new users and assist with assay development and operation of equipment.

Hours of operation are approximately 8:00 AM to 6:00 PM, Monday to Friday for outside users. Contact the facility manager for appointments, rates or more information about services.

INSTRUMENTATION

• Becton Dickinson LSRII Cell Analyzer with 4 lasers:
  • 405 nm Violet
  • 488 nm Blue
  • 561 nm Yellow-Green
  • 633 nm Red

• Becton Dickinson FACSVantage SE Cell Sorter with DiVa Option with 4 lasers:
  • 405 nm Violet
  • 488 nm Blue
  • 561 nm Yellow-Green
  • 633 nm Red

• Becton Dickinson FACSAria IIU SORP Cell Sorter with 5 lasers:
  • 355 nm UV
  • 405 nm Violet
  • 488 nm Blue
  • 561 nm Yellow-Green
  • 640 nm Red

Automated Cell Deposition Unit for sorting 96-well plates is available

• iCys Laser Scanning Cytometer with 3 lasers;
  • 405nm Violet
  • 488nm Blue
  • 633nm Red

For analysis of cells in tissue slices or cells grown on microscope slides or multi-well dishes.

SERVICES

• Training in use of equipment
• Consulting on experimental design
• Operation of instruments

Tissue culture facilities are available for maintenance of cells during experiments.