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Pavel Osten

Associate Professor
M.D., Medical School of Charles University, Prague, 1991
Ph.D., SUNY Downstate Brooklyn, 1995


Contact
osten@cshl.edu
(516) 367-6990 (p)
 
To understand what’s going wrong in illnesses like autism and schizophrenia, we need to know more about how neural circuits are connected in the healthy brain. We’ve developed advanced imaging methods to draw the first whole-brain activation map in the mouse.  Now we’re applying that technology to study changes in brain activity in mice whose behavior models human autism and schizophrenia. 

Pavel Osten’s lab works on identification and analysis of brain regions, neural circuits, and connectivity pathways that are disrupted in genetic mouse models of autism and schizophrenia. Osten hypothesizes that (1) systematic comparison of multiple genetic mouse models will allow determination of overlaps in pathology—neural circuit endophenotypes—responsible for the manifestation of neuropsychiatric disorders and (2) neural circuit-based classification of autism and schizophrenia will provide key circuit targets for detailed mechanistic studies and therapeutic development. Osten and colleagues have developed the first systematic approach to the study of neural circuits in mouse models of psychiatric diseases, based on a pipeline of anatomical and functional methods for analysis of mouse brain circuits. An important part of this pipeline is high-throughput microscopy for whole-mouse brain imaging, called serial two-photon (STP) tomography. This year, they used this method to describe the first whole-brain activation map representing social behavior in normal mice. They are currently focusing on using this approach to study brain activation changes in two mouse models of autism: the 16p11.2 df/+ mouse model, which shows an increased propensity to seizures and hyperactivity, and the CNTNAP2 knockout mouse model, which shows abnormal social behavior.

Kim, Yongsoo and Venkataraju, Kannan Umadevi and Pradhan, Kith and Mende, Carolin and Taranda, Julian and Turaga, Srinivas C and Arganda-Carreras, Ignacio and Ng, Lydia and Hawrylycz, Michael J and Rockland, Kathleen S and Seung, H.  Sebastian and Osten, Pavel (2015) Mapping Social Behavior-Induced Brain Activation at Cellular Resolution in the Mouse. Cell Reports 10(2) pp. 292-305.

Takada, N. and Pi, H. J. and Sousa, V. H. and Waters, J. and Fishell, G. and Kepecs, A. and Osten, P. (2014) A developmental cell-type switch in cortical interneurons leads to a selective defect in cortical oscillations. Nature Communications 5pp. 5333.

Osten, P. and Margrie, T. W. (2013) Mapping brain circuitry with a light microscope. Nat Methods 10(6) pp. 515-23.

Ragan, T. and Kadiri, L. R. and Venkataraju, K. U. and Bahlmann, K. and Sutin, J. and Taranda, J. and Arganda-Carreras, I. and Kim, Y. and Seung, H. S. and Osten, P. (2012) Serial two-photon tomography for automated ex vivo mouse brain imaging. Nature Methods 9(3) pp. 255-258.

Grinevich, V. and Kolleker, A. and Eliava, M. and Takada, N. and Takuma, H. and Fukazawa, Y. and Shigemoto, R. and Kuhl, D. and Waters, J. and Seeburg, P.H. and Osten, P. (2009) Fluorescent Arc/Arg3.1 indicator mice: A versatile tool to study brain activity changes in vitro and in vivo. Journal of Neuroscience Methods 184(1) pp. 25-36.

Additional materials of the author at
CSHL Institutional Repository